Optimizing Phosphorus Application Rate and the Mixed Inoculation of Arbuscular Mycorrhizal Fungi and Phosphate-Solubilizing Bacteria Can Improve the Phosphatase Activity and Organic Acid Content in Alfalfa Soil

Alfalfa (Medicago sativa L.) is an important legume forage, and phosphorus a significant nutrient element of alfalfa with high quality yield. However, the effect mechanism different application rates on soil bacteria, efficiency are still unclear. Therefore, we present results study in which was inoculated Funneliformis mosseae, Bacillus megaterium, double mosseae given no inoculation bacteria. P2O5 applied under condition bacterial inoculation, contents were 0, 50, 100, 150 mg kg?1, respectively, to explore alkaline phosphatase, organic acid, pH, matter, relationship between indicators soil, rates. The mixed significantly higher than that non-inoculated control group (p < 0.05). matter content rhizosphere non-rhizosphere soil. When rate 100 phosphatase megaterium better single inoculation. Principal component analysis showed top three treatments were: bacteria treatment kg?1 >; 50 kg?1. In addition, when addition could increase promote metabolism root exudates, acid compared without application. At same time, activity had positive correlation malic oxalic acetic total thereby improving fertility promoting absorption by plants. These findings provide new insights into soils effects additions nutrients.